Selexis CHO Cells in Suspension-1

White papers and articles

Selexis enables biopharmaceutical companies to produce virtually any recombinant therapeutic protein, because we have the ability to understand and modify our cells to address productivity and expression challenges.

Drug Discovery News Special Report: Well CHOsen?

NOVEMBER 4, 2018

Special Report on Cell BiologyWell CHOsen?Ever-more complicated biologics push technical boundaries

By Randall C Willis

“Increasingly, we see scientists addressing complicated and intractable diseases by developing complex protein therapeutics such as bi- and tri-specific proteins, DARPins, triabodies, novel scaffolds decorated with peptides, enzymes and growth factors,” says Igor Fisch, CEO of Selexis. “While this can be great news for patients, these complex molecules are often more challenging to manufacture, as most are non-natural proteins that need to be expressed at high enough levels to be commercially viable….

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Obtaining the Optimal Clone: Exploiting metabolic selection in mammalian cells for clonal enrichment, particularly for difficult-to-express proteins.

NOVEMBER 21, 2017

The life sciences industry is increasingly addressing lifethreatening diseases with sophisticated and complex protein therapeutics, such as novel scaffolds, fusion proteins, bi-specific proteins, and cytotoxic cytokines and interferons. These next-generation therapeutics, which are often difficult-to-express proteins, have been accompanied by a new set of challenges with regard to their manufacturing. Generating manufacturing cell lines that secrete high levels of intact recombinant therapeutic proteins requires more than effective and stable integration of the recombinant gene into the manufacturing cell’s genome…

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Making the Right Choice: Transient vs. Stable Pool Expression in CHO Cells

SEPTEMBER 29, 2017

Historically, transient and stable transfections have been performed for different purposes. Transient transfections can rapidly provide reasonable amounts of recombinant proteins (mg quantities) for early evaluation of their activity and typically transient transfections were used in the screening stages of a project when potential clinical candidates were being selected. Due to the time that it took to generate stable clonal cell lines (7–12 months), they were generated only when clinical candidates had been selected. Over the past 20 years, however, technological advances in the generation of stable and transient transfectants have improved productivity and reduced timelines, leading to some debate over whether it is better to use transiently or stably transfected cell lines during development.

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Keywords: clonal cell lines chinese hamster ovary cells transient transfection expression pools SUREtechnology Platform CHO cells