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CHO Fed-Batch Strategies To Rapidly Increase mAb Titer By 100% Without Sacrificing Product Quality

November 17, 2022

In the field of therapeutic antibody production, diversification of fed-batch strategies is flourishing in
response to the market demand. All manufacturing approaches tend to follow the generally accepted dogma
of increasing titer since it directly increases manufacturing output. While titer is influenced by the biomass
(expressed as IVCD), the culture time and the cell specific productivity (qp), we changed independently each
of these parameters to tune our process strategy towards adapted solutions to individual manufacturing
To do so, we worked separately on the increase of the IVCD as high seeding fed-batch capacity. Yet, as
intensified fed-batch may not always be possible due to limited facility operational mode, we also separately
increased the qp with the addition of specific media additives. Both strategies improved titer by 100% in 14
days relative to the standard fed-batch process with moderate and acceptable changes in product quality
Since intensified fed-batch could rival the cell-specific productivity of a conventional fed-batch, we developed
novel hybrid strategies to either allow for acceptable seeding densities without compromising productivity, or
alternatively, to push the productivity the furthest in order to reduce timelines.


Keywords: CHO Intensified fed-batch ambr15 2022