Scientific Publications

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Donec ac odio tempor orci dapibus ultrices in iaculis nunc. Vitae sapien pellentesque habitant morbi tristique senectus. 

1 min read

Exploring the limits of conventional small‑scale CHO fed‑batch for accelerated on demand monoclonal antibody production

November 15, 2021

In the field of therapeutic antibody production, diversification of fed-batch strategies is flourishing in response to the market demand. All manufacturing approaches tend to follow the generally accepted dogma of increasing titer since it directly increases manufacturing output. While titer is...

Read More
Keywords: CHO cell culture Intensified fed-batch ambr15 Productivity Biomass
1 min read

Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies

May 31, 2018

Native flexibly linked (NFL) HIV-1 envelope glycoprotein (Env) trimers are cleavage-independent and display a native-like, well-folded conformation that preferentially displays broadly neutralizing determinants. The NFL platform simplifies large-scale production of Env by eliminating the need to...

Read More
Keywords: recombinant protein expression HIV-1 immunogenicity viral fusion proteins adjuvants vaccines
1 min read

MAR-Mediated Transgene Integration into Permissive Chromatin and increased expression involve an SD-MMEJ-like DNA repair Pathway.

October 3, 2016

Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous recombination (HR) DNA repair pathways....

Read More
1 min read

MAR-Mediated Transgene Integration into Permissive Chromatin and increased expression involve an SD-MMEJ-like DNA repair Pathway.

August 29, 2016

Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous recombination (HR) DNA repair pathways....

Read More
Keywords: recombinant protein expression DNA recombination immunoglobulin production chinese hamster ovary cells microhomology-mediated end-joining
1 min read

Use of human MAR elements to improve retroviral vector production.

December 14, 2015

Retroviral vectors have many favorable properties for gene therapies, but their use remains limited by safety concerns and/or by relatively lower titers for some of the safer self-inactivating (SIN) derivatives. In this study, we evaluated whether increased production of SIN retroviral vectors can...

Read More
1 min read

Epigenetic regulatory elements: recent advances in understanding their mode of action and use for recombinant protein production in mammalian cells.

July 10, 2015

Successful generation of high producing cell lines requires the generation of cell clones expressing the recombinant protein at high levels and the characterization of the clones’ ability to maintain stable expression levels. The use of cis-acting epigenetic regulatory elements that improve this...

Read More
1 min read

Epigenetic regulatory elements: Recent advances in understanding their mode of action and use for recombinant protein production in mammalian cells.

June 23, 2015

Successful generation of high producing cell lines requires the generation of cell clones expressing the recombinant protein at high levels and the characterization of the clones’ ability to maintain stable expression levels. The use of cis-acting epigenetic regulatory elements that improve this...

Read More
Keywords: Epigenetics Matrix attachment region Recombinant protein production Transgene silencing Ubiquitously-acting chromatin opening element
1 min read

CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion.

January 21, 2014

The ability to efficiently produce recombinant proteins in a secreted form is highly desirable and cultured mammalian cells such as CHO cells have become the preferred host as they secrete proteins with human-like post-translational modifications. However, attempts to express high levels of...

Read More
1 min read

MAR elements and transposons for improved transgene integration and expression.

April 30, 2013

Reliable and long-term expression of transgenes remain significant challenges for gene therapy and biotechnology applications, especially when antibiotic selection procedures are not applicable. In this context, transposons represent attractive gene transfer vectors because of their ability to...

Read More
1 min read

Rapid Production of Functional Proteins of a Combinatorial IgG Library in CHO Cells

January 4, 2012

Recombinant DNA (rDNA) technologies provide a wide range of tools for producing a broad array of recombinant proteins. Since the early 1970s, the biotechnology industry has harnessed those tools — together with genetic engineering and genomics — for developing new classes of innovative and...

Read More
1 min read

High-level transgene expression by homologous recombination-mediated gene transfer.

August 15, 2011

Gene transfer and expression in eukaryotes is often limited by a number of stably maintained gene copies and by epigenetic silencing effects. Silencing may be limited by the use of epigenetic regulatory sequences such as matrix attachment regions (MAR). Here, we show that successive transfections...

Read More